Ethyl Acetate Extracts of Semen Impatientis Inhibit Proliferation and Induce Apoptosis of Human Prostate Cancer Cell Lines through AKT/ERK Pathways

Tao Wang,Yang Cai,Shaogang Wang,Wei Peng,Ruibao Chen,Weiming Yang,Junfeng Zhang,Wen Song,Yufeng Ding,Lu Liu,Dunmei Hu,Jianhan Ye,Jihong Liu

doi:10.1155/2017/1243515

Tao Wang, Yang Cai + Show 11 more

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https://doi.org/10.1155/2017/1243515

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Journal: BioMed Research International	Publication Date: Jan 1, 2017
Citations: 3	License type: CC BY 4.0

Affiliation: Tongji Hospital

Abstract

Objective. To investigate the inhibitory effect of ethyl acetate extracts of Impatiens balsamina L. on prostate cancer cells. Methods. Impatiens balsamina L. was extracted to get water, ethanol, oil ether, ethyl acetate, and butanol extracts. CCK-8 assay was used to detect the inhibitory effect. Apoptosis rates and cell cycle distribution were detected by flow cytometry. Transwell assay was performed to test the ability of migration. The expressions of Bcl-2, Bax, cleaved-caspase-3, p-ERK, ERK, p-AKT, AKT, cyclin D1, cyclin E, and MMP2 were detected by Western blot. Results. Ethyl acetate extracts had the strongest inhibitory effect. After being treated with different concentrations of ethyl acetate extracts, the percentage of G0/G1 phase increased significantly, cyclin D1 and cyclin E expression decreased, apoptosis rate was significantly higher, and the ability of migration of PC-3 and RV1 was inhibited significantly. Western blot showed that the expressions of Bcl-2, p-ERK, and p-AKT were significantly decreased, but the expressions of Bax and caspase-3 cleavage were increased. Conclusions. Impatiens balsamina L. inhibited the proliferation of human prostate cancer cells; ethyl acetate extracts have the strongest effect. It could inhibit cell proliferation and migration, cause G1 phase arrest, and induce apoptosis probably through inhibition of the AKT and ERK pathways.

Highlights

Prostate cancer is the most frequent prevalent cancers and the second highest cause of mortality by cancer for the male population in western countries [1] and has a relatively low incidence in Asia
Cell Counting Kit-8 (CCK-8) assay was applied to evaluate the antiproliferation effect of Semen Impatientis extracts on human prostate cancer cells in vitro
The results showed that Semen Impatientis extracts had different degrees of antiproliferative activity on human prostate cancer cells, while ethyl acetate extracts of Semen Impatientis (EAESI) had the strongest inhibitory effect on the growth of PC-3, RV1, and LNCaP cells (IC50 = 32, 69 and 85 μg/ml, respectively), compared to the other extracts of Semen Impatientis. (Figure 1) And EAESI significantly inhibited the growth of PC-3, RV1, and LNCaP cells in a dose-dependent manner (P < 0.05)

Summary

Introduction

Prostate cancer is the most frequent prevalent cancers and the second highest cause of mortality by cancer for the male population in western countries [1] and has a relatively low incidence in Asia. The incidence and mortality data in Asia had a remarkable increase in the past 30 years [2]. For early and localized PCa, surgery, hormone ablation, and radiation therapy are the most commonly utilized treatments. For advanced and metastasized tumors, chemotherapy is the only choice, but it has limited efficacy and could lead to various side effects in patients. It is very necessary and urgent to develop a new agent for the treatment of prostate cancer. Growing evidences suggest that many herbal medicines possess anticancer activity and are considered as an important alternative to the conventional treatments for cancers [3]

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