Ethephon and auxin induce mycorrhiza‐like changes in the morphology of root organ cultures of Mugo pine

Abstract

The effects of ethylene and auxin on the morphology and anatomy of root organ cultures of Pinus mugo Turra var. mugo were investigated to test the hypothesis that changes in root morphology associated with formation of ectomycorrhizae may be related to ethylene produced by ectomycorrhizal fungi or by host plant roots in response to fungus‐produced auxin. Morphological changes characteristic of mycorrhizal infection include dichotomous branching of lateral roots, inhibition of root hair formation and enlargement of cortical cells. Lateral roots on non‐mycorrhizal root organ cultures, grown in a defined medium, underwent dichtotomous branching while root hair formation was inhibited in response to the ethylene released by 50 and 100 μM ethephon (2‐chloroethylphosphonic acid), but no effect on cortical cell dimensions was observed. The auxin, naphthaleneacetic acid (1 and 10 μM) also stimulated dichotomous branching and inhibited root hair formation, but to a lesser extent and with a greater lag time than ethephon. Auxin‐stimulated ethylene production by root organ cultures was demonstrated. This appeared to be responsible, at least in part, for the auxin‐induced dichotomous branching since the ethylene action inhibitor, silver thiosulfate (0.1 mM) inhibited the response to auxin by 35%.