Abstract
Introduction: Garlic is a natural herb which can be used to be a good alternative treatment because cheap and safe. Garlic contains allicin which may has act antibacterial and antiinflammatory effect. Moreover, garlic extract has a good biocompatibility and can stimulate cell growth. Does garlic extract biocompatible and can stimulate cell growth that is seen from the proliferation of human gingival fibroblasts and how its work will be studied.Objective: The aim of this study was to analyze the biocompatibility of garlic extract by observing the viability and proliferation of human gingival fibroblasts in vitro.Methods: Biocompatibility test was conducted using serial concentration of garlic extract. Human gingival fibroblasts was seeded into 96 microwell plate with density of 2x103 cells, added with the fourteen serial concentration of garlic extract, and incubated in 37o C and 5% CO2for 24, 48 and 72 hours. MTT assay was used to analyze the viability and proliferation of human gingival fibroblasts. Data were analyzed by the Kruskal Wallis and U Mann-Whitney test.Results: The result showed that in each time of observation, there is no significant difference in viability fibroblast (p>0,05), but there are significant difference between time of observation at 24, 48, and 72 hours (p <0.05).Data showed that all concentration of garlic extract increased the viability and proliferation of human gingival fibroblasts.Conclusions: The ethanolic garlic extract has a good biocompatibility to human gingival fibroblasts culture cell and can stimulate the proliferation of human gingival fibroblast.Bangladesh Journal of Medical Science Vol.17(4) 2018 p.556-561
Highlights
Garlic is a natural herb which can be used to be a good alternative treatment because cheap and safe
This study analized the effect of garlic extract to viability of human gingival fibroblasts in vitro, and resulted that human gingival fibroblasts have survived after treated with garlic extracts
1).The highest viability of human gingival fibroblasts was determined in group treated with garlic extract 1.25 μg/ml at 48 hours (145.15%), and the lowest viability of human gingival fibroblasts was determined in group treated with garlic extract 0,625 μg/ml at 72 hours (79.90%)
Summary
Garlic is a natural herb which can be used to be a good alternative treatment because cheap and safe. Does garlic extract biocompatible and can stimulate cell growth that is seen from the proliferation of human gingival fibroblasts and how its work will be studied. Extensive caries area can lead to pulp exposure and it lead to pulp necrotic.[2,3] Necrotic deciduous teeth should be treated to maintain teeth for mastication so that optimal nutrition for growth and development of children can be provided..[4] The endodontic or root canal treatment has been performed to bacterial elimination and reinfection protection This treatment will restore teeth in the jaw for longtime, and maintain their function to support periodontal tissues health and free of pain.[5,6] The endodontic or root canal treatment is performed with root canal irrigation, antibacterial agent, or root canals dressing. Some dressing materials are commonly used in pediatric dentistry is calcium hydroxide and cresophene, but it is necessary to study assessed both clinically and in vitro, as well as how they affect dental periapical tissues.[7]
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