Ethanolic extracts of Moringa oleifera leaves inhibit biofilm formation of Vibrio alginolyticus in vitro

Abstract

Vibriosis caused by Vibrio alginolyticus infection is getting challenging to treat as the bacteria harbor the ability to form biofilm. One of the natural products that might be potentially applied to treat vibriosis through biofilm deformation is the leaves of Moringa oleifera. The objective of the study was to determine the effect of ethanol extracts of M. oleifera to inhibit V. alginolyticus biofilm formation in vitro. A microtiter plate biofilm assay (OD570nm) method was applied in this study. The treatments were the addition of ethanolic Moringa leaves extracts at different concentrations of 2%, 4%, 8% and 10%. The result of this study showed that Moringa leaves extract significantly inhibited the biofilm formation of V. alginolyticus. The optimal condition to inhibit biofilm formation was at 10% concentration for 60 hours incubation at room temperature signifying the potential application of Moringa leaf extracts to treat biofilmmediated diseases in aquaculture.