Ethanolic extract of Taiwanofungus camphorates enhanced cisplatin/doxorubicin induced cytotoxicity on human hepatocellular carcinoma cells

Abstract

Purpose: To develop antiasthmatic agent, Illicium verum and its major components were evaluated on their suppression effect in the ariway inflammation. Furthermore we have studied the molecular mechanism of trans-anethole compound concerning Treg cell mediated suppression. Methods: Asthma was induced in BALB/c mice by systemic sensitization to ovalbumin (OVA) followed by intratracheal, intraperitoneal, and aerosol allergen challenges. Illicium verum and its major components were orally administered for 4 weeks. We investigated their effects on airway hyperresponsiveness, pulmonary eosinophilic infiltration, various immune cell phenotypes, cytokine & cytospin measurements in Bronchoalveolar lavage (BAL), Th2 cytokine production, OVA-specific IgE production, Th1/Th2 cytokine production, lung histology in this mouse model of asthma. Results: Illicium verum and trans-anethole significantly (p<0.05) inhibited OVA-induced increases in total cell counts, eosinophil counts, and IL-4, IL-5, IL-13, and eotaxin levels recovered in bronchoalveolar lavage fluid in OVA-sensitized mice. Trans-anethole further substantially (p <0.05) reduced the total IgE, eotaxin 2 levels, andCCR3 expression of BALfluid. Trans-anethole also substantially (p <0.05) increased the IL-10, IFNlevel, and IL-10 or TGF-1 mRNA expression of BAL fluid. Histological studies showed that TRANS-ANETHOLE dramatically inhibited eosinophilia, and infiltration of lymphocytes in lung tissues Conclusion: These result suggest that the antiinflammatory and anti-asthmatic effects of Illicum verum and trans-anethole may be exerted through upregulation of regulatory T cells. Contact: Seung-Hyung Kim, sksh518@dju.kr