Ethanolic extract of local sorghum seedcoat (Sorghum bicolor [L.] Moench) as a potential bacterial growth inhibitor

Abstract

Research on the antibacterial activity of ethanol extract from seedcoat of local Sorghum (Sorghum bicolor [L.] Moench) against Staphylococcus aureus and Escherichia coli has been conducted. The purpose of this study was to determine the minimum inhibitory concentration of extract against both gram-positive and negative bacteria. The extraction method used in this study was a maceration method using ethanol as a solvent. Antibacterial activity test was carried out with several concentrations, namely 1 g/mL, 0.8 g/mL, 0.6 g/mL, 0.4 g/mL, 0.2 g/mL, 0.1 g/mL, 0.05 g/mL, 0.025 g/mL, and 0.01 g/mL. The results showed that the extract inhibited bacterial growth in a wide range of concentrations. The smallest inhibition was at a concentration of 0.05 g/mL with a clear zone diameter of 10.29 mm in Staphylococcus aureus and 11.70 mm in Escherichia coli. Ethanol extract of local Sorghum seedcoat exhibited a strong inhibition ability and probably a potential antibacterial agent source.