Ethanolic extract of Crinum asiaticum attenuates hyperglycemia-mediated oxidative stress and protects hepatocytes in alloxan induced experimental diabetic rats

Abstract

The present study is to investigate the antioxidant activity in alloxan induced diabetic rats. The experimental rats were randomly divided into three groups: Group I: control; Group II: alloxan induced diabetic rats and Group III: alloxan induced diabetic rats were treated with ethanolic extract of Crinum asiaticum leaves (200mg/kg/bw). Diabetes mellitus was induced by alloxan in a single dose of 200mg/kg/bw. A significant decrease in blood sugar level was observed and also an increase in high density lipoprotein (HDL) level and a decrease (P<0.05) in cholesterol, triglyceride, low density lipoprotein (LDL) and very low density lipoprotein (VLDL) levels were observed after 15days of treatment with C. asiaticum extract. Besides, the activities of aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), acid phosphatase (ACP) and lactate dehydrogenase (LDH) (P<0.05) were diminished in the C. asiaticum leaves extract of the supplemented group. In addition, the activity of superoxide dismutase (SOD), catalase (CAT) and reduced glutathione (GSH) (P<0.05) was increased by the C. asiaticum leaves extract administration and increases in lipid peroxides (LPO) in liver were found to be ameliorated after treatment. The findings of the present investigation demonstrated the hepatocyte protective nature of C. asiaticum by attenuating markers of hyperglycemia-mediated oxidative stress and the antioxidant competence in hepatic tissues of diabetic rats.