Ethanolic Extract of Citrus Sinensis Peel Markedly Mitigate Paracetamol-Induced Hepatotoxicity in Rats

Abstract

Background: Herbal medicines have historically been crucial for enhancing human health. Previous studies have highlighted the antioxidant properties of various parts of Citrus sinensis. In light of widespread reliance on plant-based remedies, this study focuses on a particular herbal medicine, Citrus sinensis, known for its rich content of vitamin C, pectins, phenolic compounds, and flavonoids. Objective: This research aims to investigate the hepatoprotective potential of the ethanolic extract of Citrus sinensis peel in vivo, particularly focusing on its antioxidant capabilities. Methods: The study utilized biochemical parameters including aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), bilirubin, and total proteins to assess the hepatoprotective action of the Citrus sinensis peel extract in serum of Wistar albino rats. The rats were divided into five groups, with varying treatments including a control group, a paracetamol-induced hepatotoxicity group, and groups receiving different doses of the Citrus sinensis extract. Results: Treatment with the ethanolic extract of Citrus sinensis peel at doses of 200 mg/kg and 400 mg/kg significantly mitigated the paracetamol-induced elevation of serum levels of AST, ALT, ALP, bilirubin, and total proteins. These improvements were comparable to those observed with the standard drug, silymarin. Conclusion: The study confirms the hepatoprotective effects of Citrus sinensis peel extract against paracetamol-induced liver damage. This protection was evident both biochemically and histopathologically, suggesting the potential therapeutic utility of Citrus sinensis in liver disorders.