Abstract
We investigated whether abrupt withdrawal from chronic ethanol (EW) provokes mitochondrial aging. Five, 12, and 16 month‐old female rats consumed 6.5% ethanol for 3 mo, and then were abruptly withdrawn from the diet to impose EW stress. Mitochondria were isolated from cerebellum harvested at 24 h EW. Control rats consumed an isocaloric dextrin diet. Mitochondrial membrane swelling (MMS), an indicator of mitochondrial membrane fragility was assessed by measuring light absorbance of mitochondrial suspensions at 540 nm, and reported (Table: means ± SEM, n = 5–6) as time (min) to 50% decline in absorbance. While the control diet did not affect MMS at any age, EW accelerated MMS (*P < 0.05 vs. control diet), especially in the oldest rats. These results show that EW sensitizes mitochondrial membranes to aging in a manner that is more prominent in old than young rats. Mitochondrial membranes appear to be the target of a deleterious interaction between EW and aging brains of female rats. (Support: NIAAA/AA013864, NIAAA/AA015982).
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