Abstract
This work examined the simultaneous saccharification and fermentation (SSF) process for the biological conversion of pulp & paper sludge (PPS) and monosodium glutamate waste liquor (MGWL) into ethanol using Saccharomyces cerevisiae CICC 1001 in batch condition. At the SSF conditions of sterilization, pH 6.0, 6% TS (total solid), and adding nutrient solution, the highest ethanol yield of 42.5gL−1 was obtained at the enzyme loading of 40A.U. (activity unit)g−1VSfed (volatile solid); only if the pH value was changed to 4.5, the ethanol yield reduced by 47%; only if the TS concentration was changed to 3%, the ethanol yield decreased by 11%; only if the substrates were not sterilized, 5% decrease occurred to the ethanol production; only if no nutrient solution was added to the reactor, 97% reduction of ethanol yield happened; the extent of influence of the impact factors ranked in the order of nutrient solution>pH>TS concentration>sterilization. The Fourier Transform Infrared (FTIR) spectra of substrates after SSF further verified the results of process performances. Therefore, this SSF of PPS and MGWL reduced the residue streams and land-filling costs while benefiting in ethanol production.
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