Abstract

BackgroundAs the most abundant renewable resources, lignocellulosic materials are ideal candidates as alternative feedstock for bioethanol production. Cassava residues (CR) are byproducts of the cassava starch industry which can be mixed with lignocellulosic materials for ethanol production. The presence of lignin in lignocellulosic substrates can inhibit saccharification by reducing the cellulase activity. Simultaneous saccharification and fermentation (SSF) of furfural residues (FR) pretreated with green liquor and hydrogen peroxide (GL-H2O2) with CR saccharification liquid was investigated. The final ethanol concentration, yield, initial rate, number of live yeast cells, and the dead yeast ratio were compared to evaluate the effectiveness of combining delignificated lignocellulosic substrates and starchy substrates for ethanol production.ResultsOur results indicate that 42.0 % of FR lignin removal was achieved on FR using of 0.06 g H2O2/g-substrate and 9 mL GL/g-substrate at 80 °C. The highest overall ethanol yield was 93.6 % of the theoretical. When the ratio of 0.06 g/g-H2O2-GL-pretreated FR to CR was 5:1, the ethanol concentration was the same with that ratio of untreated FR to CR of 1:1. Using 0.06 g/g-H2O2-GL-pretreated FR with CR at a ratio of 2:1 resulted in 51.9 g/L ethanol concentration. Moreover, FR pretreated with GL-H2O2 decreased the concentration of byproducts in SSF compared with that obtained in the previous study.ConclusionsThe lignin in FR would inhibit enzyme activity and GL-H2O2 is an advantageous pretreatment method to treat FR and high intensity of FR pretreatment increased the final ethanol concentration. The efficiency of ethanol fermentation of was improved when delignification increased. GL-H2O2 is an advantageous pretreatment method to treat FR. As the pretreatment dosage of GL-H2O2 on FR increased, the proportion of lignocellulosic substrates was enhanced in the SSF of the substrate mixture of CR and FR as compared with untreated FR. Moreover, the final ethanol concentration was increased with a high ethanol yield and lower byproduct concentrations.

Highlights

  • As the most abundant renewable resources, lignocellulosic materials are ideal candidates as alternative feedstock for bioethanol production

  • furfural residues (FR) was pretreated with various concentrations of H2O2, as shown in Fig. 1, with 9 mL Green liquor (GL)/g-dry substrate for 3 h at 80 °C and pH 12.0

  • After the green liquor and hydrogen peroxide (GL-H2O2) pretreatment, the solid yield was reduced with increasing amounts of H2O2, ranging from 79.0 to 75.0 % (Fig. 1)

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Summary

Introduction

As the most abundant renewable resources, lignocellulosic materials are ideal candidates as alternative feedstock for bioethanol production. Cassava residues (CR) are byproducts of the cassava starch industry which can be mixed with lignocellulosic materials for ethanol production. There is currently an upsurge of interest in the search for renewable biomass to produce liquid transportation fuels such as bioethanol. This interest has been resulted from environmental concerns about toxic-gas emissions. FR is an industrial byproduct from corncobs-based furfural production [4]. Cellulose and lignin in the cobs are relatively stable under furfural production conditions. Utilization of FR to produce ethanol would be effective in the dispose of waste products, while reducing environmental pollution

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