Abstract
The TPNH dependent oxidation of ethanol by rat liver microsomes has been studied. Microsomes isolated by the usual centrifugation techniques contain TPNH oxidase, catalase and alcohol dehydrogenase (ADH). The latter two enzymes, known to be involved in ethanol metabolism, appear to be present largely as contaminants and their activity can be removed or decreased by washing. No evidence was obtained for the presence of a separate and distinct membrane bound ethanol oxidase. It is concluded that the TPNH and O 2 dependent metabolism of ethanol by microsomes can readily be explained by the coupled and combined activities of TPNH oxidase, catalase and ADH.
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