Abstract
Chronic Kidney Disease (CKD) has increased incidence and prevalence in developing nations. In this in vitro study, we evaluated the cells proliferative effects, fibronectin (FN), transforming growth factor β (TGF-β1), and Reactive oxygen species (ROS) - level inhibition potential of ethanol extract of detam 1 soybean seed (EEDS) on glucose-induced kidney mesangial cells (SV40 MES 13). The cells proliferation assay used 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)2H-tetrazolium (MTS) assay. FN and TGF-β1 level were measured using ELISA assay kit and ROS level using flow cytometry. Level of FN, TGF-β1 and ROS, on CKD cells model (5 mM, 10 mM glucose-induced mesangial cell) treated with EEDS 6.25 µg/mL were lower significantly compared to positive control, EEDS improve cells viability and decrease FN, TGF-β1 and ROS level in glucose-induced kidney mesangial cells as CKD cells model.
Highlights
Chronic Kidney Disease (CKD) is a condition when a renal injury occurs, resulting ina decreased of renal excretory function, causing an accumulation of waste and metabollic products
Kidney mesangial cells cultured under high glucose conditions provide a model system for studying the effect high glucose on FN and collagen IV (COL4) (Miller et al, 2014), transforming growth factor-β (TGF-β) deposition (Widowati et al, 2018; Prahastuti et al, 2019a)
The glucose-induced mesangial cell free supernatant without extract was used as positive control
Summary
Chronic Kidney Disease (CKD) is a condition when a renal injury occurs, resulting ina decreased of renal excretory function, causing an accumulation of waste and metabollic products. These products accumulations in blood and organs will cause various complications. Kidney mesangial cells cultured under high glucose conditions provide a model system for studying the effect high glucose on FN and collagen IV (COL4) (Miller et al, 2014), transforming growth factor-β (TGF-β) deposition (Widowati et al, 2018; Prahastuti et al, 2019a). Merr ethanol extract (EEDS) for CKD therapy by measuring the cells proliferation effect, FN, TGF-β1, and ROS level on glucose-induced kidney mesangial cells (SV40 MES 13)
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