Ethanol effects on bradykinin-stimulated phosphoinositide hydrolysis in NG 108-15 neuroblastoma-glioma cells

Abstract

The effect of short- and long-term ethanol exposure on bradykinin-stimulated hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP 2) was investigated in neuroblastoma × glioma hybrid cells (NG 108-15). Acute exposure of 50–150 mM ethanol neither influenced the bradykinin-stimulated accumulation of [ 3H]-inositol phosphates (IP 1, IP 2, IP 3) nor the hydrolysis of PIP 2 in cells labelled with [ 3H]-inositol. Furthermore, ethanol (100 mM) added in the absence of agonist did not influence these parameters. However, in cells cultivated for 4 days in 100 mM ethanol, PIP 2 hydrolysis and IP 1, IP 2 and IP 3 formation after stimulation by 10 −6–10 −5 M bradykinin was markedly inhibited while there was no effect on the basal levels or on the levels found after stimulation with low concentrations of bradykinin. The inhibitory effect of ethanol on IP accumulation became significant after 2–3 days of ethanol.