Et Özgüllüğünün Belirlenmesinde Primer Setlerinin Tasarımına Yönelik Biyoinformatik Tabanlı Bir Yaklaşım

Abstract

Polymerase chain reaction (PCR) and its derivatives are one of the most widely used DNA-based methods in species determination studies in meat and meat products. Chromosomal or mitochondrial genes of the species can be targeted in PCR-based analyzes used in species detection studies. Many researchers are able to realize oligonucleotide differences between species through online alignment programs on mitochondrial DNA. Using chromosomal DNA would provide more concise results in quantification studies. However, determining the marker regions for genomic DNA is challenging due to the large size of the chromosomes. Bioinformatics approaches are available for selected applications. However, using those approaches requires intensive knowledge of computer science, molecular biology, and bioinformatics in addition to high computational power. In this study, a pipeline is presented that will provide a user-friendly approach to be adopted by facilities where contamination analyzes are routinely performed.