Abstract
The morphological study of blood cells is considered as the basis of several studies, since the shape of the cell, its nucleus arrangement and the level of chromatin condensation are easy to note, as well as detecting pathologies that cause blood abnormalities. The aim of this study was to evaluate the morphology of blood cells found in the qualitative count of the species Piaractus brachypomus. There were analysed cells from 43 fishes, with an average weigth of 1.34 Kg and a mean length of 39.23 cm, in a total of 43000 cells were observed, thus it was possible to verify the ocurrence of erythrocyte, thrombocytes and six types of leukocytes: lymphocytes, basophils, eosinophils, neutrophils, heterophiles and monocytes. In addition, it was noticed toxic neutrophils and a small number of morphological nuclear changes such as micronuclei and invaginations In general the blood cells of P. brachypomus presented morphological similarities when compared to the morphology of the bone fish cells.
Highlights
The morphological study of blood cells is considered as the basis of several studies, since the shape of the cell, its nucleus arrangement and the level of chromatin condensation are easy to note, as well as detecting pathologies that cause blood abnormalities
Foi realizada a colheita de sangue com seringa de 5 ml sem anticoagulante, em vasos localizados na região caudal conforme metodologia proposta por Ueda et al (1997) e Ranzani-Paiva et al (2013)
Os exemplares de Piaractus brachypomus apresentaram peso médio de 1,34 ± 0,32 kg e comprimento total de 39,23 ± 2,42 cm
Summary
O presente trabalho foi submetido e aprovado na Comissão de Ética no Uso de Animais (CEUA) da Universidade Federal Rural da Amazônia (UFRA), sob o protocolo 005/2017. Foi realizada a colheita de sangue com seringa de 5 ml sem anticoagulante, em vasos localizados na região caudal conforme metodologia proposta por Ueda et al (1997) e Ranzani-Paiva et al (2013). Um total de 86 lâminas (duas para cada peixe) foram preparadas. As lâminas foram coradas com panótico rápido LB, metodologia estabelecida por Romanowsky, na qual a extensão hematológica é submetida à ação de um fixador e duas soluções corantes, por meio de imersões de 5 segundos em cada, e ao final da última imersão encontra-se pronta para leitura, onde estruturas basófilas coram de azul, azurófilas (ricas em peroxidase) coram de púrpura e acidófilas de rosa.
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