ESTUDO FITOQUÍMICO E POTENCIAL ANTIBACTERIANO DO LÁTEX DE Himatanthus drasticus (MART.) PLUMEL

Abstract

Himatanthus drasticus (Mart.) Plumel, popularly known as janaguba, is a latex medicinal plant widely used in the region of Cariri (CE) and used as an alternative source for the treatment of numerous diseases. The objective of the present work was to test the antibacterial and modulatory potential of the latex in natura (LIHD) and ethyl acetate extract of H. drasticus latex (EAEHD) alone and in combination with aminoglycoside antibiotics (amikacin and gentamicin) against standard and multiresistant strains, following the method of microdilution in Brain Heart Infusion (BHI) broth. In the evaluation of the minimum inhibitory concentration (MIC) results were obtained ≥ 1024 μg / mL against the standard strains of Escherichia coli , Staphylococcus aureus , Pseudomonas aeruginosa and Klebsiella pneumoniae for both LIHD and EAEHD. The natural products presented synergism in the activity of the aminoglycosides to strains of multiresistant bacteria Escherichia coli (EC 27), Staphylococcus aureus (SA 358) and Klebsiella pneumoniae (KP 10031), however they presented antagonism to Pseudomonas aeruginosa (PA 03). In the quantification of phenols, LIHD had 62.6 mg / g and EAEHD 51.8 mg / g gallic acid / g extract, and for total flavonoids, LIHD had 16.5 and EAEHD had 13.4 mg / g of flavonoids. Further research is needed for a possible use of these natural products combined with antimicrobials tested (aminoglycosides) against pathogenic strains. Through the results it was concluded that the natural products represent promising sources in the fight against bacterial resistance. Keywords: Aminoglycosides; Antimicrobian activity; Inhibitory Minimum Concentration; Himatanthus drasticus .