Abstract
The present study describes the results of preliminary phytochemical screening, Toxicological Artemia salina bioassay and antimicrobial activity in Bauhinia forficata link, known for popularly Pata-de-vaca. The Bauhinia is a pantropical genus, its species are used in the treatment of several infections and diseases, especially diabetes. The phytochemical prospection used crude ethanolic extract (EBE) of the stem bark of Bauhinia forficata Link; the toxicity analyses in Artemia salina for given LC 50% . For the evaluation of the antimicrobial activity was used the method of diffusion-disk with strains of Klebsiella pneumoniae (ATCC13883), Staphylococcus aureus (ATCC 25923) and Escherichia coli (ATCC 25922) and standard antibiotics, EBE was used at concentrations of 25mg/mL , 50mg/mL and 100mg/mL. Observed in the phytochemical screening the presence of reducing sugars, Depsideos and Depsidonas, phenols and tannins, anthraquinones and flavonoids. For cytotoxicity LC 50% was 853.80 mg / mL with low toxicity. To test the antimicrobial no inhibition was observed at any concentration. The presence of some classes of secondary metabolites corroborates in part to the activities alleged by the population, mainly flavonoids, which have hypoglycemic action, more studies to confirm this biological activity must be performed. Keywords: Pata-de-vaca; phytochemical screening; toxicology test; Antibacterial Action.
Highlights
The present study describes the results of preliminary phytochemical screening, Toxicological Artemia salina bioassay and antimicrobial activity in Bauhinia forficata link, known for popularly Pata-de-vaca
P.; GOSMANN, G. et al Farmacognosia: da planta ao medicamento
Summary
Inúmeros grupos de produtos naturais possuem atividade antimicrobiana, sendo assim é necessária à avaliação frente a bactérias de patogenicidade elevada, para que se possa comprovar tal atividade. Após secagem por 3 a 4 minutos, os discos (papel filtro Whatman – tipo 3) impregnados com o extrato bruto etanóico das cascas do caule três concentrações diferentes (25, 50 e 100μg/mL) foram aplicados sobre o ágar. De 6 mm de diâmetro, impregnados com cada concentração do EBE das cascas do caule, foram aplicados sobre as placas com o auxílio de pinça flambada e fria, sendo pressionados para melhor aderir ao meio. 3. Resultados Através do screening fitoquímico ou prospecção fitoquímica do extrato etanóico das cascas do caule da espécie B. forficata Link foi possível identificar a presença de Flavonóides, Fenóis e Taninos, Depsídeos e Depsidonas, Açucares Redutores e Antraquinonas (Tabela 1).
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