Abstract
Ozonation of theobroma fat at different applied ozone dosages was carried out and peroxide indexes along with antimicrobial activity were determined. The reaction products were identified using Proton Nuclear Magnetic Resonance Spectroscopy ( 1 H NMR). The principal signal intensity values were used for following the reaction course between ozone and theobroma fat. The reaction was followed up to peroxide index values of 544 mmol-equiv/kg. The intensities of olefinic proton signals decreased with the gradual increase in ozone concentration until disappearing completely. The Criegee ozonides obtained at 87.4 mg/g of ozone doses were approximately 3.7 times higher than at the beginning of the reaction. The highest action spectrum of antimicrobial activity was obtained with the higher peroxide index. These results suggest that the 1 H NMR spectroscopic technique provides valuable information about of the ozonization grade of theobroma fat which produces an affective antimicrobial activity against Candida albicans
Highlights
La grasa de teobroma ha sido utilizada ampliamente en las industrias alimenticia y farmacéutica como materia prima en la elaboración de bombones y supositorios vaginales, debido a que su punto de fusión de (33-35 °C) se encuentra muy cercano a la temperatura del cuerpo humano. (B.P., 2000a; Saavedra, 1997).Al reaccionar el ozono con las grasas y aceites vegetales se forman una serie de productos oxigenados como peróxidos, ozónidos, aldehídos, ácidos carboxílicos entre otros (Bailey, 1978)
These results suggest that the 1H NMR spectroscopic technique provides valuable information about of the ozonization grade of theobroma fat which produces an affective antimicrobial activity against Candida albicans
Antibacterial activity of ozonized sunflower oil (Oleozon)
Summary
Study of ozonized theobroma fat using Proton Nuclear Magnetic Resonance and microbiological analysis. The reaction products were identified using Proton Nuclear Magnetic Resonance Spectroscopy (1H NMR). The principal signal intensity values were used for following the reaction course between ozone and theobroma fat. The intensities of olefinic proton signals decreased with the gradual increase in ozone concentration until disappearing completely. The Criegee ozonides obtained at 87.4 mg/g of ozone doses were approximately 3.7 times higher than at the beginning of the reaction. The highest action spectrum of antimicrobial activity was obtained with the higher peroxide index. These results suggest that the 1H NMR spectroscopic technique provides valuable information about of the ozonization grade of theobroma fat which produces an affective antimicrobial activity against Candida albicans. KEY-WORDS: 1H NMR - Microbiology - Peroxides index - Ozone - Ozonized theobroma fat
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