Estudio comparativo de la morfología de sangre periférica analizada mediante el microscopio y el CellaVision DM96 en enfermedades hematológicas y no hematológicas

Abstract

Introduction Differential leukocyte counts of peripheral blood cells are an important diagnostic tool. Objective We evaluated the CellaVision DM96 (CellaVision AB, Lund, Sweden), an automated system for digital peripheral blood cell analysis. Material and methods We analysed 234 blood films in which leukocyte values were from 1.12 to 282 × 10 9/L. A total of 177 blood films were from patients with hematological diseases. Results Correlation coefficients between results obtained from the CellaVision DM96 pre-classification and by conventional direct microscopy were excellent for segmented neutrophils, lymphocytes, monocytes and blasts (r > 0.87 < 0.94 and P < .0001) and good for band neutrophils, eosinophils, basophils and plasma cells (r > 0.74 < 0.81 and P < .0001). After the reclassification of the cells, very good concordance coefficients were observed for promyelocytes and myelocytes (> 0.7), intermediate for reactive lymphocytes and erythroblasts (>0.5 and < 0.7) and low (<0.5) for metamyelocytes. Whatever the pathology and the number of blasts on the films, all 97 patients were positive for blast detection on the DM96. Pathological cells such as prolymphocytes, large granular lymphocytes, hairy cells, Sézary cells and other atypical lymphocytes were reclassified by the user. Conclusions Advantages of the CellaVision DM96 over direct microscopy include, requires less time than manual differentiation, is a good tool for educational purposes, improve the traceability of the results, and can have an important role in a modern Hematology Laboratory.