Abstract
Abstract Searching for the associations between the gene polymorphism and the reproductive traits is essential in defining the genetic native breed specificity, which distinguishes them from the other breeds. The aim of our study was to determine the associations between mutations in the PRL, PRLR, PTGS2, FUT1 genes and sexual and periparturient activity in native Pulawska gilts. The analysis included 72 animals which gave birth to the first litter. Evaluation of the productive value of gilts accounted for indicators of sexual and periparturient activity as well as reproductive traits. The biological material for molecular analyses was obtained from the hair roots of the gilts. The genotype was verified by PCR RFLP analysis. The primers and PCR conditions were determined on the basis of available literature data. Statistically significant differences (P≤0.05) were found at the PRL locus: gilts of AA genotypes (Ins/Ins) at the PRL locus were characterised by longest farrowing duration compared to gilts of AB genotype (P≤0.05). The analysis of PRLR gene showed that gilts of TT genotype revealed a tendency for later occurrence of estrus signs (first and second estrus) and for the markedly longest farrowings (P≤0.05). With regard to PTGS2 and FUT1 loci, no significant differences were found in the parameters of sexual and periparturient activity of the gilts. However, gilts of FUT1 GG genotype gave birth to and reared the largest first litters (P≤0.05). The results of the studies expand the knowledge about the genetic structure and productivity of Pulawska gilts.
Highlights
Prostaglandin-endoperoxide G/H synthase-2 (PTGS2), known as cyclooxygenase 2, is involved together with cyclooxygenase 1 in the conversion of arachidonic acid to prostanoids, i.e. prostaglandin H (PGH2) (Vane et al, 1998)
The aim of the study was to determine the associations between prolactin gene (PRL), prolactin receptor gene (PRLR), PTGS2, and FUT1 genes polymorphisms and reproductive traits in native Pulawska gilts
Our study showed no statistically significant effect of the polymorphism of this gene on age at first farrowing in Pulawska gilts, and on indicators of sexual activity during first and second estrus
Summary
Prostaglandin-endoperoxide G/H synthase-2 (PTGS2), known as cyclooxygenase 2, is involved together with cyclooxygenase 1 in the conversion of arachidonic acid to prostanoids, i.e. prostaglandin H (PGH2) (Vane et al, 1998). The prostaglandins synthesised in the presence of COX play key roles in the reproductive system, e.g. during ovulation, fertilisation, embryo implantation and parturition, which has caused PTGS2 to be recognised as a gene with a potentially large impact on reproductive traits in animals (Ding et al, 2006; Sugimoto et al, 2015). The FUT1 gene is considered a potential marker for resistance to edema disease in pigs (Bao et al, 2012; Wang et al, 2012) and for reproductive performance traits of sows (Horak et al, 2005). The results of research for reproductive performance are discrepant. The aim of the study was to determine the associations between PRL, PRLR, PTGS2, and FUT1 genes polymorphisms and reproductive traits in native Pulawska gilts
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