Estrogens in seminal plasma of human and animal species: Identification and quantitative estimation by gas chromatography—Mass spectrometry associated with stable isotope dilution

Abstract

Estrone, 2-methoxyestrone and estradiol-17β have been definitively identified in seminal plasma of man, bull, boar and stallion by high resolution gas chromatography associated with selective monitoring of characteristic ions of suitable derivatives. Quantitative estimations were performed by isotope dilution with deuterated analogues and by monitoring molecular ions of trimethylsilyl ethers of labelled and unlabelled compounds. Concentrations of unconjugated and total estrogens are reported together with the statistical evaluation of accuracy and precision.