Abstract
DNA-dependent DNA polymerase activity in the immature rat uterus has been examined in terms of molecular species present and their response to estrogen treatment. The molecular species of DNA polymerase were characterized by DEAE-cellulose chromatography, sucrose density gradient analysis, sulfhydryl reagent sensitivity and optimal assay conditions. DNA polymerase α activity was found predominantly in the high-speed cytosol but was also present in 0.4 M KCl nuclear extract preparation. DNA polymerase β activity was only observed in nuclear extracts. Estradiol treatment resulted in a dose-dependent, estrógen-specific increase in DNA polymerase activity, principally due to increases in cytoplasmic DNA polymerase α activity. A 3–4-fold maximal increase of DNA polymerase activity occurred 24–30 h after a single hormone treatment in close correspondence to hormone-induced increased DNA synthesis. When hormone treatment was sustained by daily injections of estradiol, the DNA polymerase response became refractory to continued stimulation after two days.
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