Abstract
Immunohistochemical staining for estrogen receptor (ER) was performed on :12 infiltrating duct carcinomas of the breast using formalin-fixed, paraffin-embedded sections, monoclonal anti-ER (H222, Abbott Laboratories) and a strepavldin-biotin peroxidase method. Results of ER staining in paraffin sections were compared with those obtained on frozen sections and cytosolic ER levels obtained by a dextran-coated method on the same cases. Twenty-seven of the tumours were positive for ER in paraffin sections with a concordence of 94% between staining in paraffin sections and In frozen tissue (r = 0.76; p <0.0001) and 97% between staining in paraffin sections and cytosolic values (r = 0.60; p <0.001). The percentage of positively stained tumour cells in paraffin sections varied from 0 to 94% with staining intensities which were comparable with that obtained in frozen sections. This study validates the immunohistochemical demonstration of ER in formalin-fixed, paraffin-embedded breast carcinomas.
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