Abstract
The expression of the proto-oncogene c- jun in response to estradiol treatment in immature and mature rat uterine tissue was measured using a cDNA encoding the mouse c- jun proto-oncogene. This probe hybridized to a major RNA band of 2.7 kb and a minor 3.2 kb band. In Northern blots of total RNA from both immature and mature rat uteri, estradiol treatment resulted in at least a 3 fold increase in expression of the 2.7 kb band over control levels by 3 hr post injection. By 12 hr post injection, expression of c- jun mRNA had returned to control levels. A strong induction (>5 fold) of c- jun mRNA expression was also observed in stroma-myometrial tissue isolated from mature rats approximately 3 hours after treatment with estradiol. The similar kinetics of induction of c- fos and c- jun emphasizes the functional significance of the fos/ jun heterodimer in control of uterine cell proliferation.
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