Abstract
The levels of albumin mRNA in Xenopus laevis liver were measured at various times after injection of estradiol using two different methods involving hybridization of cloned albumin cDNA to total liver RNA. The absolute levels of albumin mRNA fell by more than 95% during the first 4 days following estrogen treatment, then slowly returned to normal levels over the following 12 days. Albumin synthesis paralleled the albumin mRNA levels during the first 8 days after injection; but, 16 and 32 days after injection, albumin synthesis again decreased while albumin mRNA remained at normal levels. The time courses of the effects of estrogen on albumin and vitellogenin mRNA levels were different. Whereas albumin mRNA levels were minimal 4 days after estradiol injection, vitellogenin mRNA levels were maximal 8 days after injection.
Highlights
Were measured at various times after injeocftieosntra- Animals-Male X . laeuis (-60 g) were injected with 1 mgof diol using two different methods involving hybridiza- estradiol dissolved in propylene glycol
The albumin mRNA levels in untreated animals varied over a 3-fold range (13,600-55,600 molecules/cell)
The stringency of hybridization in the present approach may be somewhat lower than previously usedin the R,t analysis, we obtain the sameamount of albuminmRNA
Summary
Were measured at various times after injeocftieosntra- Animals-Male X . laeuis (-60 g) were injected with 1 mgof diol using two different methods involving hybridiza- estradiol dissolved in propylene glycol. Laeuis (-60 g) were injected with 1 mgof diol using two different methods involving hybridiza- estradiol dissolved in propylene glycol They were maintained on a tion of cloned albumin cDNA to total liver RNA. The livers were weighed and total cytoplasmic RNA extracted as mRNA levels during thefirst 8 days after injection; but, described previously (10). Albumin or vitellogenin mRNA levels were estimated by the amount of nick-translated cDNA which hybridized to the immobilized RNA. Standard curves were constructed the liver (7, 8) It is difficult, to evaluate whether using known amounts of purified vitellogenin mRNA (0-100 ng) or there was an absolute decrease in albumin synthesisin these albumin mRNA The levels of the 68K and 74K albumins and of total vitellogeninin the plasma of estrogen-treated X . laevis were measured by scanning the gels
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