Year-end Sale % OFF 
Abstract
Administration of 17β-estradiol to reproductively active male rainbow trout results in the induction of synthesis of two estrogen-responsive mRNA, vitellogenin (VG) and ULER2, to high levels. The complementary DNA (cDNA) of these two mRNA were prepared by molecular cloning. Two genomic DNA fragments generated by EcoRI digestion of trout genomic DNA showed hybridization to VG cDNA. A quantitative RNA dot blot hybridization method was adopted for the determination of levels of estrogen-responsive mRNA in livers of male trout after 17β-estradiol stimulation. This assay was used to determine the dose-response relationship and the time course of estrogen-responsive mRNA accumulation in juvenile fish.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
