Abstract

High-affinity, low-capacity estrogen-specific binding sites were detected in nuclei isolated from hagfish liver by the use of an in vitro [H3]estradiol exchange assay. A single class of high-affinity sites was found in highest concentration in liver from adult vitellogenic female fish. Nonvitellogenic females had a small amount of estrogen binding by liver nuclei. No estrogen binding could be detected in nuclei from either liver tissues of a sexually immature hagfish or muscle tissues of vitellogenic adults. The results suggest that estrogen induction of vitellogenesis in hagfish may be mediated by nuclear hormone receptors.

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