Abstract

Estrogen binding protein (EBP) in mammary tumor was studied in DMBA induced rat mammary carcinoma, Shionogi carcinoma 115, Shionogi carcinoma 42 and human mammary tumor by use of 3H-estradiol.Free and bound estradiol was separated by Sephadex G-25.Specific EBP was calculated 4.5S by sucrose gradient centrifugation. Non-specific EBP was observed in the 3.2S-3.6S region of the ultracentrifugal analysis.Specific EBP was migrated to anodic region of α- or β-position in disc electrophoresis.

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