Abstract
A radioimmunoassay technique has been used to measure unconjugated estriol, estriol sulfate, and estriol glucosiduronate. The distribution of these compounds in plasma, blood cells, and interstitial fluid has been studied. It was found that in the plasma roughly two thirds of each constituent was bound to protein. All three forms also occur in the interstitial fluid but in much lower concentration. The proportion of estriol in interstitial fluid not bound to protein was found to be larger than in plasma. Plasma volumes were measured and it was calculated that the total estriol contained in the interstitial fluid was somewhat more than in plasma. Small amounts of each form of the steroid were associated with the cellular elements in blood, the proportions being larger in the case of unconjugated estriol and estriol sulfate than with estriol glucosiduronate.
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