Estradiol binding in the cytosol and nuclear pellet of electrolytically treated rat uteri

Abstract

Intact mature rat uteri positioned in saline buffer at 0–2°C between platinum electrodes operating at a voltage in the range 1–4 VDC, with periodic reversal of polarity, are conditioned so that the cytosols obtained from these uteri lose 40% of specific and 32% of non-specific estradiol binding capacity and 90% of the dissolved protein. By contrast, estradiol bound in the nuclear pellet following incubation of the uterus in solution of the hormone shows a 50% increase in quantity if the uterus was electrolytically pretreated. The results are regarded as reflecting the removal from intact tissue by electrolytic treatment of a substance that inhibits and controls the translocation of estradiol receptor from the cytoplasm to the nucleus.