Abstract
ObjectiveTo analyze the efficiency of high voltage pulsed current (HVPC) with early application in three different sites, in the regeneration of the sciatic nerve in rats submitted to crush injury, the sciatic functional index (SFI) was used to assess the functional recovery.MethodsAfter crushing of the nerve, 57 animals were submitted to cathodal HVPC at frequency of 50Hz and voltage of 100V, 20 minutes per day, 5 days per week. The rats were divided into five groups: control group; ganglion group; ganglion + muscle group; muscle group; and sham group. The SFI was determined weekly for seven weeks, from the preoperative period to the 6th postoperative week.ResultsCompared with the control group, the results showed a significantly better performance of group 2 for the first 3 weeks; group 3 showed significantly better performance in the third week; and group 4 showed a significantly negative performance during the 4th and 6th weeks.ConclusionEarly application of HVPC had a positive effect in the treatment of the spinal cord region and the sciatic nerve root ganglion with a dispersive electrode on the contralateral lumbar region or on the gastrocnemius. However, HVPC had a negative effect in the treatment with an active electrode on the gastrocnemius and a dispersive electrode on the contralateral thigh. Level of evidence II, Prospective comparative study.
Highlights
Experiments on recovery after peripheral nerve injuries involve various techniques, both to produce the injury, and in terms of interventions that seek to accelerate the regeneration process.[1,2,3] The two most common ways of producing this type of injury are transection and crushing
A crush injury is a useful modality for the study of peripheral nerve regeneration, as it mimics a type of axonotmesis, leading to distal Wallerian degeneration, but with a good prognosis of functional recovery
Hypothesis: high voltage pulsed current (HVPC) would be efficient in accelerating the regeneration of the sciatic nerve of rats after crush injury
Summary
Experiments on recovery after peripheral nerve injuries involve various techniques, both to produce the injury, and in terms of interventions that seek to accelerate the regeneration process.[1,2,3] The two most common ways of producing this type of injury are transection and crushing. On the other hand, when using a device developed to produce the crush injury, it is possible to control weight and time, knowing the severity of the injury that is being produced.[4,5] A crush injury is a useful modality for the study of peripheral nerve regeneration, as it mimics a type of axonotmesis, leading to distal Wallerian degeneration, but with a good prognosis of functional recovery Support structures such as the satellite cell, the basement membrane and the supporting connective tissue are preserved.[6,7] There is injury to the vessels that supply blood for the vital functions of the nerve.[8] The sciatic nerve of several animals, especially that of rats, is widely used in a large number of experiments for analysis of motor and sensory function.[1,9,10]. Hypothesis: HVPC would be efficient in accelerating the regeneration of the sciatic nerve of rats after crush injury
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