Abstract
The most probable numbers (MPN) of spirilla were estimated in samples of Cladophora, pond water and mud, stream water and slime, trickling filter effluent, standing hay, field drainage, cow manure, woods soil, and cultivated field soil. Six formulations of MPN media were compared: vermiculite–albumin, nutrient gelatin agar, calcium lactate, artificial activated sludge, 5-fluorouracil, and hay infusion. Vermiculite–albumin generally gave the greatest numbers of three morphological types of spirilla. Spirilla were about 4% of the total bacterial population associated with Cladophora; about 0.1 to 0.6% of the bacteria of mud, water, slime, trickling filter effluent, and cow manure; and less than 0.01% of the remainder except hay, in which they were not found.
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