Estimation of Zagorsky carps genetic variability using microsatellite analysis

Abstract

Six microsatellite dinucleotide loci were used to study the genetic variability in three generations of Zagorsky carp. All investigated loci are polymorphic with number of alleles from 4 to 9 per locus. The part of heterozygotes at microsatellite alleles in this breeding group was high, on the average more than 87%. Genetic variability of Zagorsky carp last selective generations has been revealed to be kept high, but it stay lower than that of Moscow Scaly and Moscow Mirror carps (intrabreed types of Parsky breed). Sixth and seventh generations showed somewhat less genetic variability than fifth generation did, in this connection further control of its level is desirable. The loss of 2 alleles from two microsatellite loci, MFW1 and MFW9, was found at sampling of Zagorsky carp seventh generation; these alleles occurred in both preceding generations. 18% of Zagorsky carps had only one of two amplification products (additional) at MFW31 locus, whereas the other carp groups had both products or hadn’t additional product in rare samples. No specific alleles for genetic marking of this breeding group have been found at six microsatellite loci. Allele sizes at all these loci in Zagorsky carp group and in other examined carp groups lay in the same length ranges.