Abstract
An assay is described based on a high pressure liquid chromatography system for measurement of vitamin D 3 and 25-hydroxyvitamin D 3 concentration in adipose tissue and muscle. The sensitivity of the assay is < 1 ng/g of tissue. Neither vitamin D 3 nor 25-hydroxyvitamin D 3 were found in tissues of vitamin D-deficient rats using this method. 25-Hydroxyvitamin D 3 could not be-detected in adipose tissue and very little was found in muscle of normal rats. The mean vitamin D 3 concentrations in 15 samples of human perirenal adipose tissue was 45.3 ng/g ± 22.2 (SD) and in 6 samples of axillary tissue 115.6 ng/g ± 52.4 (SD). Human adipose tissue contains a substantial amount of vitamin D 3 and its contribution to the maintenance of vitamin D status is discussed.
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