Estimation of the hemoglobin glycation rate constant

Masashi Kameyama,Masafumi Koga,Tsuyoshi Iwasaka,Hirotaka Matsui,Toshika Okumiya,Yoshihisa Matsumura,Kazuyuki Hiratani,Yasuhiro Ono,Shinji Tokuhiro

doi:10.1038/s41598-020-80024-7

Abstract

In a previous study, a method of obtaining mean erythrocyte age (M_{RBC}) from HbA1c and average plasma glucose (AG) was proposed. However, the true value of the hemoglobin glycation constant (k_g dL/mg/day), required for this model has yet to be well characterized. Another study also proposed a method of deriving M_{RBC} from erythrocyte creatine (EC). Utilizing these formulae, this study aimed to determine a more accurate estimate of k_g. One hundred and seven subjects including 31 patients with hemolytic anemia and 76 subjects without anemia were included in this study. EC and HbA1c data were analyzed, and M_{RBC} using HbA1c, AG and the newly-derived constant, k_g were compared to M_{RBC} using traditional ^{51}hbox {Cr} in three patients whose data were taken from previous case studies. A value of 7.0times 10^{-6} dL/mg/day was determined for k_g. M_{RBC} using HbA1c, AG and k_g were found to no be significantly different (paired t-test, p=0.45) to M_{RBC} using traditional ^{51}hbox {Cr}. k_g enables the estimation of M_{RBC} from HbA1c and AG.

Highlights

In a previous study, a method of obtaining mean erythrocyte age ( MRBC ) from HbA1c and average plasma glucose (AG) was proposed
HbA1c is generally indicative of recent glycemic control over the past 1–2 months, it is known to show reduced correlation to glycemic control status in the presence of diseases which result in a shortened erythrocyte lifespan such as hemolytic anemia[3]
We have recently proposed a simple method to obtain mean erythrocyte age ( MRBC ) from HbA1c and average glucose (AG)[6], which has theoretically derived based on Ŵ-like function model of erythrocyte lifespan[7]: HbA1c

Summary

Introduction

A method of obtaining mean erythrocyte age ( MRBC ) from HbA1c and average plasma glucose (AG) was proposed. The true value of the hemoglobin glycation constant ( kg dL/mg/day), required for this model has yet to be well characterized. Another study proposed a method of deriving MRBC from erythrocyte creatine (EC). Utilizing these formulae, this study aimed to determine a more accurate estimate of kg. Abbreviations AG Average plasma glucose CGM Continuous glucose monitoring DM Diabetes mellitus EC Erythrocyte creatine GA Glycated albumin Hb Hemoglobin HPLC High performance liquid chromatography Hpt Haptoglobin IFCC International Federation of Clinical Chemistry kg Hemoglobin glycation constant MCDP 10-N-methylcarbamoyl-3,7-bis (dimethylamino) phenothiazine MRBC Mean erythrocyte age NGSP National Glycohemoglobin Standardization Program Ret Reticulocyte SMBG Self-monitoring of blood glucose. HbA1c is generally indicative of recent glycemic control over the past 1–2 months, it is known to show reduced correlation to glycemic control status in the presence of diseases which result in a shortened erythrocyte lifespan such as hemolytic anemia[3]

Objectives

Methods

Conclusion