Abstract
Aim. To study the effects of lithium salts on production of cytokines by immunocompetent cells in the whole-blood culture of patients with alcohol dependence and affective disorders.Materials and methods. The study materials were blood samples from 25 patients with alcohol dependence (AD) and 12 patients with bipolar disorder (BD). Blood diluted 1:1 with complete RPMI-1640 medium (Gibco, UK) was added to the wells of the culture plate, then new lithium salts (succinate, fumarate, pyruvate, ascorbate) and a reference salt – lithium carbonate at a final concentration of 1.2 mmol / l per lithium ion – were added. In parallel, control samples without lithium salts were tested; the samples were incubated for a day. The concentration of cytokines (interferon (IFN) γ, interleukin (IL)-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-17A, tumor necrosis factor (TNF) α) was determined in the culture supernatants on the MAGPIX multiplex analyzer (Luminex, USA) (Center for Collective Use “Medical Genomics”, Tomsk NRMC) using the Human Cytokine / Chemokine Magnetic Bead Panel (Merck, Germany).Results. All lithium salts had a unidirectional effect on the production of cytokines by immunocompetent cells (ICC), except for lithium ascorbate and IL-8. The concentrations of cytokines in the supernatants of loaded and control samples (spontaneous production) were comparable, which indicates an absence of stimulating or suppressing effects of salts on the functional activity of ICC under the experimental conditions. The effect of lithium ascorbate as an IL-8 inducer was detected: the production of IL-8 induced by lithium ascorbate was 2.3–2.5 times higher than its spontaneous production.Conclusion. The obtained results, as well as the previously revealed antioxidant and cytoprotective properties of new lithium salts, confirmed that they are promising for development of pharmacological agents with combined action.
Highlights
Control samples without lithium salts were tested; the samples were incubated for a day
All lithium salts had a unidirectional effect on the production of cytokines by immunocompetent cells (ICC), except for lithium ascorbate and IL-8
The effect of lithium ascorbate as an IL-8 inducer was detected: the production of IL-8 induced by lithium ascorbate was 2.3–2.5 times higher than its spontaneous production
Summary
Оценка влияния солей лития на продукцию цитокинов клетками крови в опытах in vitro Ветлугина Т.П.1, Епимахова Е.В.1, Савочкина Д.Н.1, Плотников Е.В.1, 2, Бойко А.С.1, Иванова С.А.1, Бохан Н.А.1. Цель – изучить влияние солей лития на продукцию цитокинов иммунокомпетентными клетками в культуре цельной крови больных с алкогольной зависимостью и аффективными расстройствами. Все соли лития оказывали однонаправленное действие на продукцию спектра цитокинов иммунокомпетентными клетками (ИКК) за исключением аскорбата лития и ИЛ-8. Полученные результаты, а также выявленные ранее антиоксидантные, цитопротекторные свойства новых солей лития подтверждают их перспективность для разработки фармакологических средств комбинированного действия. Оценка влияния солей лития на продукцию цитокинов клетками крови. Для цитирования: Ветлугина Т.П., Епимахова Е.В., Савочкина Д.Н., Плотников Е.В., Бойко А.С., Иванова С.А., Бохан Н.А. Оценка влияния солей лития на продукцию цитокинов клетками крови в опытах in vitro. Vetlugina T.P.1, Epimakhova E.V.1, Savochkina D.N.1, Plotnikov E.V.1, 2, Boiko A.S.1, Ivanova S.A.1, Bokhan N.A.1
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