Abstract
DNA-based molecular markers such as Inter Simple Sequence Repeat (ISSR), Sequence-Related Amplified polymorphism (SRAP) and Random Amplified Polymorphic DNA (RAPD) were used in this study to examine the genetic differences among sixteen Iraqi wheat varieties. Seventy three primers out of 177 were reproducible and showed clear amplified bands. The degree of genetic diversity, Polymorphism information content (PIC) and resolving power (RP) were estimated. All the studied molecular markers were informative and showed good ability to classify and distinguish 16 wheat varieties. Total number of polymorphic bands is 134, 221 and 55 for ISSR, SRAP and RAPD respectively. PIC and RP values were 0.259, 0.264 and 0.262 and 9.06, 7.87 and 2.78 Original Research Article
Highlights
Wheat (Triticum aestivum L.) is one of the most important cereals in the world and Iraq
DNA-based molecular markers such as Inter Simple Sequence Repeat (ISSR), Sequence-Related Amplified polymorphism (SRAP) and Random Amplified Polymorphic DNA (RAPD) were used in this study to examine the genetic differences among sixteen Iraqi wheat varieties
It is necessary to investigate the genetic diversity in wheat germplasm to understand and in the future broaden the genetic variation available for breeding
Summary
Wheat (Triticum aestivum L.) is one of the most important cereals in the world and Iraq. It is necessary to investigate the genetic diversity in wheat germplasm to understand and in the future broaden the genetic variation available for breeding. For effective conservation and use of genetic resources, evaluation of genetic variation within collections could be dramatically enhanced by using DNA molecular markers. These DNA markers, when closely linked to genes of interest can be used to select for the desirable allele/s in a marker assisted breeding programs [4]. Many scientists have investigated the genetic diversity in wheat crop using different molecular markers such as random amplified polymorphic DNA (RAPD), restriction fragment length polymorphism (RFLP), amplified fragment length polymorphism (AFLP), and inter simple sequence repeat (ISSR). The goal of this study is to evaluate different DNA-based makers tehnologies for their ability to estimate the genetic diversity among 16 different Iraqi wheat varieties
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