Abstract
An estimation procedure of the radial heterogeneity of the distributions of the mobile phase flow velocity and of the local column efficiency was derived from a comparison of experimental data and of the characteristics of these tailing peaks calculated numerically. The analysis of these characteristics indicated that the radial heterogeneity of the packing density of a column is a cause of peak tailing. This analysis showed also that it is possible to correlate the radial fluctuations of the flow velocity and the apparent efficiency of different columns. The comparison of experimental data and calculated results suggested also a correlation between the radial distributions of the flow velocity and of the column efficiency. Previous experimental data concerning the radial heterogeneity of column packings validated the correlation. Information on the degree of heterogeneity of the radial distributions of the flow velocity and the column efficiency were obtained by analyzing the peak width and the asymmetry factor of elution peaks. It was also found that the exponentially modified Gaussian distribution cannot provide a comprehensive representation of all types of tailing profiles in chromatography.
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