Abstract
The present investigation was carried out during two successive summer seasons of years 2019 and 2020 at the Faculty of Agriculture (Saba Basha), Alexandria University and the laboratory of the vegetable seed of Sabahya Horticulture Research Station, Alexandria Governorate, Egypt to evaluate six local cultivars and landraces of cowpea for some morphological characters, yield and its components as well as estimate some genetic parameters. Results reflected obvious differences among the six genotypes of cowpea for most of the studied characters. The coefficient of variation (C.V.) was less than 10 % for all the studied traits in all genotypes of cowpea. These results indicate that the six genotypes of cowpea are genetically identical concerning these traits. Analysis of variance showed that variances of genotypes were highly significant in all studied traits. These findings refer to that there were highly variations between genotypes under study. Generally, the data prove that all of the studied traits could be improved through the selection method, but with different degrees of the improving depending upon the amount of variation present in each population. Meanwhile, mean squares of years were significant only in height of the first flower, this can be interpreted as this property being affected by the different environmental conditions in both years of the study. Cluster analysis, based on RAPD plus ISSR analysis, divided the 6 studied genotypes into 3 major groups. The first contained Geza and Kareem7 Cvs. with similarity of (30%), the second consisted of Fowa Lr. and Kaha Cv., and the third one contained the ones of Behira Lr. and Kafr Elshikh Cv.
Highlights
According to FAOSTAT (2019), cowpea (Vigna unguiculata (L.) Walp.), 2n=22, is one of the most widely grown legume crops
Estimation of the Coefficient of Variation and Some Genetic Parameters of Some Landraces of Cowpea 21. This investigation was aimed to study the coefficient of variation and genetic differences within and between 6 different genotypes of cowpea as a first step including them in breeding programs to improve and/or establish new cultivars
PCR based on RAPD and Inter Simple Sequence Repeat (ISSR) Analysis: Genomic DNA Isolation: Genomic DNA was extracted from the young leaves of the six cow bean genotypes by using DNA extraction kits (Easy Pure Plant Genomic DNA Kit)
Summary
According to FAOSTAT (2019), cowpea (Vigna unguiculata (L.) Walp.), 2n=22, is one of the most widely grown legume crops. This investigation was aimed to study the coefficient of variation and genetic differences within and between 6 different genotypes of cowpea as a first step including them in breeding programs to improve and/or establish new cultivars. Analysis of variance in Table (5) showed that the mean square of genotypes was highly significant in all studied traits. All variance components values presented in Table (6) revealed that the large portion of genotypic variance for the following characters: plant height, height of the first flower, number of pods/plants, total pods yield/plant, total seeds yield/plant and 100 seeds weight.
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