Estimation of serum matrix metalloproteinases among patients of oral squamous cell carcinoma.

Nakhshab Choudhry,Sana Sarmad,Noor Ul Ain Waheed,Aamir Jamal Gondal

doi:10.12669/pjms.35.1.68

Abstract

Objective:To estimate the serum levels of matrix metalloproteinases in oral squamous cell carcinoma patients and in healthy subjects.Methods:In this observational study, biopsy diagnosed oral squamous cell carcinoma patients (n= 38) were recruited from Mayo Hospital, Lahore during 2016 to 2017. Age and gender matched Controls (n= 38) were also included. Venous blood sample of each participant was drawn, serum separated and the levels of matrix metalloproteinases were measured by multiplex ELISA.Results:Serum levels of MMP-1, -8, -10, -12 and -13 in OSCC patients showed statistically significant increase as compared to control group (p < 0.01). The MMP-12 predicted the presence of OSCC with highest AUC of 0.836 (95% CI [0.733 to 0.911]) for sensitivity and specificity of 80% and 78.9%, respectively for a cut-off value of 16.13 pg/ml.Conclusions:MMP-12 has been found to have significant sensitivity and specificity to qualify as a diagnostic biomarker.

Highlights

Oral Squamous Cell Carcinoma (OSCC) has the highest incidence among oral cancers globally.1
Receiver Operating Characteristic (ROC) curve analysis was performed to determine the area under the curve (AUC) with 95% confidence interval for sensitivity and specificity of the statistically significant Matrix metalloproteinases (MMPs) of both groups to evaluate the predictive value of OSCC
Among the MMPs having statistically significant increase in their values for OSCC group as compared to control group, the MMP-12 predicted the presence of OSCC with highest AUC of 0.836 for sensitivity and specificity of 80.0% and 78.9%, respectively (Fig.1)

Summary

INTRODUCTION

Oral Squamous Cell Carcinoma (OSCC) has the highest incidence among oral cancers globally. Multiple risk factors have been linked with OSCC, including such specific factors as tobacco- and alcohol-use (substance-use). Regardless of this strong association, a considerable fraction of patients develop OSCC without exposure to them, underlining the role of genetic predisposition and oncogenic viruses.[2]. Many recent studies have reported the impact of MMPs in the advancement of OSCC either directly altering the extracellular environment or indirectly through initiation of vascular regression.[5,6] Currently employed clinical tools for the detection of OSCC like biopsy, exfoliative cytology and vital tissue staining are generally applicable to a select group of patients and have distinct limitations.[7]. We measured the levels of MMP-1, -2, -3, -7, -8, -9, -10, -12, and -13 in OSCC patients and healthy subjects

METHODS

RESULTS

DISCUSSION

CONCLUSION