Estimation of serum Alpha feto-protein (AFP), interlukin-6 and Des-?-carboxyprothrombin (DCP) in case of hepatocellular carcinoma

Ali Muzaffar,Anand Sharan,Bandana Kumari,Rekha Kumari,Syed Shahzadul Haque,Uday Kumar

doi:10.3329/bjms.v15i2.19602

Ali Muzaffar, Anand Sharan + Show 4 more

Open Access

https://doi.org/10.3329/bjms.v15i2.19602

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Abstract

Background: Hepatocellular carcinoma (HCC) is one the most common primary malignancy of the liver and represents the third leading cause of cancer-related deaths worldwide. Incidence rates are highest in East Asia and Sub-Saharan Africa. A number of evidence suggests a possible role of interleukin-6 (IL-6), ?-Fetoprotein (AFP) and Des-?-carboxyprothrombin (DCP) in the pathogenesis of hepatocellular carcinoma (HCC). The high DCP may be related to increase tumour behaviour, such as the presence of vascular invasion and intrahepatic metastasis of HCC cells. Patients and methods: We studied IL-6, AFP and DCP in patients with HCC or in healthy controls. AFP was measured by chemiluminescent immunoassay; Serum IL-6 and DCP were measured by enzyme linked immunosorbent assay in 30 patients with primary hepatocellular carcinoma and 30 normal subjects.Results: IL-6, AFP and DCP were found high in the serum of patients initially diagnosed with HCC (18±9.8), (315.99±594.62) and (26.15±5.01) respectively compared with healthy subjects (4.29±2.10), (3.13±1.27) and (4.25±1.22). A significant positive correlation was found between mean levels of IL- 6 & AFP in HCC (P < 0.05), Combination of IL-6, AFP and DCP improved the sensitivity in diagnosing HCC or predicting future HCC development. Conclusions: IL-6, DCP along with AFP could be considered a promising tumor marker for HCC. DCP is a well recognized tumor marker for the screening and diagnosis of HCC. In particular, the diagnostic value of the test is significantly increased when combined with AFP.Bangladesh Journal of Medical Science Vol.15(2) 2016 p.230-233

Highlights

Hepatocellular carcinoma (HCC) most common liver cancers reported world wide
alpha fetoprotein (AFP) is a major serum glycoprotein comprised of 591 amino and 4% carbohydrate residues, encoded by a gene on chromosome 4q11-q13 with a halflife of 5-7 days, which is synthesized by fetal
Afetoprotein (AFP) and des-γ-carboxy Blood samples were collected from eighty patients prothrombin (DCP) have been widely used for who were attending to Indira Gandhi Institute of HCC diagnosis and follow-up surveillance as tumor Medical Sciences Patna teaching hospital

Summary

Introduction

Hepatocellular carcinoma (HCC) most common liver cancers reported world wide. Males are more prone to HCC [1]. 1. Dr Syed Shahzadul Haque, Biochemist, Dept of Clinical Biochemistry, Indira Gandhi Institute of Medical Sciences, Patna-14, India. 2. Dr Rekha Kumari, Asst prof , Dept of Clinical Biochemistry, Indira Gandhi Institute of Medical Sciences, Patna-14, India. 4. Dr Uday Kumar, Prof & Head, Dept of Clinical Biochemistry, Indira Gandhi Institute of Medical Sciences, Patna-14, India. 5. Dr Anand Sharan, Prof, Dept of Clinical Biochemistry, Indira Gandhi Institute of Medical Sciences, Patna-14, India. 6. Dr Bandana Kumari, Senior Resident, Dept of Clinical Biochemistry, Indira Gandhi Institute of Medical Sciences, Patna-14, India. Afetoprotein (AFP) and des-γ-carboxy Blood samples were collected from eighty patients prothrombin (DCP) have been widely used for who were attending to Indira Gandhi Institute of HCC diagnosis and follow-up surveillance as tumor Medical Sciences Patna teaching hospital. Only 15%-30% of early HCC case[9]

The study was approved by the ethical committee

Findings

The mean serum AFP level in case HCC was