Estimation of Plasma Glucose and Creatinine Level in Alloxan-induced Diabetic Wistar Rats treated with Metformin and Thiazolidinediones

Abstract

Introduction: Diabetes increases the risk of developing neuropathy. In this study, we estimate the effect of Metformin, rosiglitazone, and pioglitazone on the body weight, blood glucose and creatinine level of alloxan-induced diabetic rats. Materials and Method: Alloxan (150 mg/kg) was used to induce hyperglycemia in fasted rats. Hyperglycaemia was treated with metformin, 150 mg/kg/d; pioglitazone, 3 mg/kg/d; and rosiglitazone, 10 mg/kg/d). The anti-diabetic drugs were administered orally for 28 days. The kidneys were removed when the animals were sacrificed plasma and kidney creatinine level was measured. Results: There was the reduction in weight of diabetic rats and those administered with metformin while there was an increase in weight of rats administered with rosiglitazone and pioglitazone. There is also reduction in blood glucose level of all treated rats compared with the diabetes control. The effects of creatinine on muscle metabolism generation and serum are insignificant at P≤0.05. Conclusion: This experiment suggests that short-term diabetes does not have an effect on plasma creatinine level and muscle metabolism of alloxan-induced diabetic rats.