Estimation of osteoprotgrin level in B thalassemia patient

Abstract

Beta thalassemia is a hereditary hemolytic anemia caused by a defect in the ability of erythroblasts to synthesize the β chain of hemoglobin. The aims of the current study were to investigate the diagnostic value of serum Osteoprotegerin in B-TM patients, and assay as an early biomarker for osteoporosis. Methods: This cross-sectional study was conducted at Thalassemia Center at Al-Zahraa Teaching Hospital in Al–Najaf province, Iraq, in the period from January 2018 to July 2018. this study was conducted with eighty-eight of males that age ranges from 2-29 years, which divided in 60 of males with β-thalassemia and 28 voluntary healthy as a control groups. Assay of study estimation of parameters level that include: iron and Hb; osteoprotgrin, vitamin D3, ferritin, Hepcidin and Transferrin measurement by using sold phase enzyme linked immune sorbent assay. It also investigates the relationship between these biomarkers levels, and, Body Mass Index, and splenic status. Results: The results reported a significant (P < 0.05) decreased in Hb, body mass index, Hepcidin and vitamin D3 hormone, while serum iron, ferritin levels, Transferrin exhibited a significant (P < 0.05) increases in β-thalassemic patients as comparison with healthy groups. ELISA assay of serum Osteoprotegerin (OPG) revealed significantly higher levels for thalassemia patients than healthy controls. Of particular interest is the obvious positive correlation between OPG levels transferrin and negative correlation with serum vitamin D3. Conclusion: The current study concluded that a Osteoprotegrin is some diagnostic markers for B-thalassemia associated negatively with decrement in Vit D level.