Estimation of norovirus removal performance in a coagulation–rapid sand filtration process by using recombinant norovirus VLPs

Abstract

Norovirus (NV) is an important human pathogen that causes epidemic acute nonbacterial gastroenteritis worldwide. Because of the lack of a cell culture system or an animal model for this virus, studies of drinking water treatment such as separation and disinfection processes are still hampered. We successfully estimated NV removal performance during a coagulation–rapid sand filtration process by using recombinant NV virus-like particles (rNV-VLPs) morphologically and antigenically similar to native NV. The behaviors of two widely accepted surrogates for pathogenic waterborne viruses, bacteriophages Qβ and MS2, were also investigated for comparison with that of rNV-VLPs. Approximately 3-log 10 removals were observed for rNV-VLPs with a dose of 40 μM-Al or -Fe, as polyaluminum chloride at pH 6.8 or ferric chloride at pH 5.8, respectively. Smaller removal ratios were obtained with alum and ferric chloride at pH 6.8. The removal performance for MS2 was somewhat larger than that for rNV-VLPs, meaning that MS2 is not recommended as an appropriate surrogate for native NV. By comparison, the removal performance for Qβ was similar to, or smaller than, that for rNV-VLPs. However, the removal performances for rNV-VLPs and Qβ differed between the coagulation process and the following rapid sand filtration process. Therefore, Qβ also is not recommended as an appropriate surrogate for native NV.