Abstract
Summary A mass spectrometric 16 O 2 / 18 O 2 technique was used to determine the rate of gross O 2 evolution in attached leaves of Triticum aestivum under several environmental conditions. Simultaneously, fluorescence emission was determined from the upper and the lower sides of the leaf. The rate of non-cyclic electron flow derived from gross O 2 evolution deviated from the rates predicted from fluorescence measurements. This deviation varies depending on environmental conditions and on the side on which the leaf fluorescence is being measured. Our data clearly indicate that fluorescence measurements provide trends in electron transport but do not yield accurate electron transport rates of the entire leaf.
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