Abstract

Some modifications of the stannous chloride reduction method for the estimation of inorganic phosphate reported by Berenblum-Chain are suggested. By this method, it is possible to measure 25 μg l−1 of phosphorus /P/ as inorganic phosphate in the presence of macromolecules like deoxyribonucleic acid /100 μg ml−1/, as compared to 200 μg l−1 of P by the original method. If a larger sample volume /30 ml/ is used, even 5 μg l−1 of P can be measured.

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