Estimation of Indole Derivatives Effects in Pea Mesophyll Protoplast Suspensions

Abstract

ABSTRACTThe effects of 3—Indoleacetic acid (IAA), 3—Indolepyruvic acid (IPA), 5—Hydroxytryptamine (5—HT), 5,6—Dihydroxytryptamine (5,6—DHT) added at concentrations 10−7M to pea mesophyll protoplast suspensions were studied. It was demonstrated that IAA and 5,6-DHT decreased the surface potential of Pisum sativum cv. Ran 1 protoplasts. The ability of protoplasts to aggregate was not influenced significantly by treatment with the indole derivatives. Aggregation was not changed even if Ca2+ ions were added to the washing solutions. Pyrene test measurements of membrane fluidity of treated and untreated protoplasts did not show significant changes evoked by the indole derivatives. The experiments carried out with (14C) labelled IAA indicated that the great amount of IAA does not penetrate into the protoplasts. Studying protoplast viability after fusion procedure we found out that the number of dead protoplasts was increased by IPA and 5—HT treatment. Our results indicate that although indole derivatives tested...