Estimation of indocyanine green 15-minute retention rate using Hitachi 7180 automatic analyser

Abstract

The indocyanine green (ICG) clearance test has been used to assess the reserve of hepatic function. This method is based on the spectrometric measurement of its plasma concentration at maximum wavelength of 805nm, which requires a spectrophotometer and associated maintenance. We established an ICG measurement method using a Hitachi 7180 automatic chemistry analyser that can be tuned to a wavelength of approximately 800nm. The retention rate of ICG at 15min was determined for 41 patients using a conventional method and our method. The ICG clearance test using the automatic analyser showed excellent agreement. It is expected that the ICG test using the automatic analyser can replace the conventional ICG clearance test.