Estimation of human saliva structurization at dehydration on the solid substrate

Abstract

The aim of this work is specification of biophysical technologies in estimation of human saliva dehydration structurization. Materials and methods. We study saliva specimens by 95 healthy 24—27 years old adults. Specialties of own and initiated (by 0.1%, 0.9% and 10% solutions of sodium chloride; 0.1 N hydrochloric acid solution; 0.01 N potassium hydroxide solution) saliva structurization at dehydration on hard padding were estimated with system of morphometric parameters and spectrometry investigation. The range of estimated parameters of crystallography included: crystallizability, structure index, type of interaction of crystalline and amorphous structures, facies destruction degree, uniformity of crystal distribution, expression of cellular structure, edge zone and other facies zones. To describe teziographic facies we used: main teziographic coefficient, belts coefficient crystallinity, and the rest parameters are the same ones for crystalloscopy. We used 3 wave lengths (300, 350 and 400 nm) for spectrometry of dehydrated specimens. Role of saliva temperature, pH and osmolarity in biological fluid structurization was fixed. Results. Teziocrystalloscopic and spectrometric patterns of dehydration of samples of oral fluid of healthy humans was established according to a new system of evaluation criteria. It was stated, that saliva dehydration is dynamic process of its liquid component elimination, determined by its composition. Micro- and macro-environment factors (temperature, pH, and osmolarity) affect on saliva dehydration results considerably.